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Molecular Biology Reagents

RNA ISOLATION:

1.      RNAzol B®

        Complete and ready-to-use reagent for isolation of total RNA from samples of human, animal, plant, bacterial and viral origin. 

Cat #         Quantity

WAK-CS105      200 ml

2.      TRI Reagent®

        A  reagent for the single-step isolation of total RNA or simultaneous isolation of RNA, DNA and proteins from tissues, cells cultured in monolayer or cell pellets.1 ml of TRI Reagent processes up to 100 mg tissue or 107 cells.

Cat #         Quantity

TR 118      200 ml

3.      TRI Reagent®  BD

        A reagent specifically designed for the single-step isolation of total RNAor simultaneous isolation of RNA, DNA and proteins from whole blood, plasma or serum. 0.75 ml of TRI Reagent® BD processes 0.25 ml of blood derivatives.

Cat #         Quantity

TB 126      200 ml

4.      TRI Reagent®  LS

        A reagent for the single-step isolation of total RNA or simultaneous isolation of RNA, DNA and proteins from liquid samples such as cell suspensions, serum, CSF and amniotic fluid. 0.75 ml of TRI Reagent® LS processes 0.25 ml of liquid samples.

Cat #         Quantity

TS 120      200 ml

5.      BCP

        BCP is a molecular biology grade 1-bromo-3-chloropropane.This new phase separation reagent substitutes for highly volatileand toxic chloroform in the TRI Reagent procedures.

Cat #         Quantity

BP 151      200 ml

6.      FORMAzol®

        Stabilized formamide for use as an RNA solubilizer instead of DEPC-treated water.It allows storage of RNA at -20C, protects from degradation by Rnase,and simplifies sample preparation for northern analysis.

Cat #         Quantity

FO 121      25 ml

7.      Oligo (dT)-Cellulose Columns

        A set of 5 reusable columns and one vial of PolyAcryl Carrier for isolation of poly A+ RNA.

Cat #         Quantity

OT 125     set

8.      PolyAcryl Carrier

        Molecular biology grade acryl polymer for use in precipitation of RNA and DNA.

Cat #         Quantity

PC 152      5 ml

9.      High Salt Precipitation Solution

        For use in the TRI Reagent procedure, to eliminate polysaccharideand proteoglycan contamination from RNA samples.

Cat #         Quantity

PS 161      100 ml

10.   Phosphate Buffer for Spectrophotometry

        100X stock solution for use in the spectrophotometric analysis of RNA and DNA.

Cat #         Quantity

SP 130      100 ml

DNA ISOLATION:

1.      DNAzol®

        Genomic DNA isolation reagent for use with tissues, cells and liquids. Non-toxic, no phenol use, the isolation is fast (10-30 min), simple and reliable. 1 ml of DNAzol® isolates DNA from 25-50 mg tissue or 1-3 x 107 cells.

Cat #         Quantity

DN 127     200 ml

2.      DNAzol® ES

        Extra Strength DNAzol® reagent for isolation of genomic DNA from plants. Non-toxic. 1.5 ml DNAzol® ES isolates DNA from 0.5 g of plant sample.

Cat #         Quantity

DN 128     200 ml

3.      DNAzol®BD

        A DNAzol® reagent specifically designed for isolation of genomic DNA from whole blood. Non-toxic. 1 ml of DNAzol® BD isolates DNA from 0.5 ml of whole blood.

Cat #         Quantity

DN 129     200 ml

4.      Bactozol™ Kit

        A kit for the isolation of bacterial DNA. Kit includes 10X Bactozol™ Enzyme Solution, Bactozol Enzyme Solution Dilution Buffer and DNAzol® sufficient for 125 isolations, each yielding up to 40 µg bacterial DNA.

Cat #         Quantity

BA 154     125 reactions

5.      Bactozol™ Enzyme Solution

        A solution stable at room temperature containing activated lysozyme for the effective lysis of a broad range of bacterial specimens. Sufficient for 125 lysis reactions, each with up to 40 µg bacterial DNA.

Cat #         Quantity

BZ 160      125 reactions

ELECTROPHORESIS & HYBRIDIZATION:

1.      Methylene Blue Stain

        Staining solution for qualitative and quantitative analysis of RNA and DNAimmobilized on hybridization membranes, for use in Southern and northernblotting, detects 25 ng of RNA or DNA.

Cat #         Quantity

MB 119    500 ml

2.      Background Quencher™

        10X concentrated blocking reagent that prevents non-specificbinding of hybridization probes.

Cat #         Quantity

BQ 112     30 ml

60 ml

3.      Molecular Biology Agarose

        Suitable for electrophoretic separation of nucleic acids in molecular biology applications. RNase-free, DNase-free ultra pure agarose.

Cat #         Quantity

MA 124    100 g

200 g

4.      Super Agarose

        Aqueous agarose suspension designed for optimal electrophoretic separationof nucleic acids. This 10% stock suspension can be diluted with a variety of electrophoresis buffers. RNase-free and DNase-free.

Cat #         Quantity

SA 153      200 ml

500 ml

5.      High Efficiency Hybridization System

        A complete hybridization solution containing dextran sulfate and Background Quencher™.

A Component of Super Hyb Kit.

Cat #         Quantity

HS 114      100 ml

250 ml

500 ml

6.      High Efficiency Hybridization System - with 50% formamide

        A Component of Super Hyb Kit.

Cat #         Quantity

HS 114 F   100 ml

250 ml

500 ml

7.      Washing & Pre-Hyb Solution

        10X concentrated washing and pre-hyb solution for use in northern and Southern blotting.

A Component of Super Hyb Kit.

Cat #         Quantity

WP 117    500 ml

1000 ml

8.      Super HYBTMKIT

        A kit providing the highest signal intensity and low background in Northern, Southern and dotblot hybridizations. Contains solutions for hybridizations, pre- and post- hybridization washes.

Cat #         Quantity

SK 116-SM

SK 116-LG         10-20 hybs

25-50 hybs

9.      SUPER HYBTMKIT with 50% formamide

Cat #         Quantity

SK 116F-SM

SK 116F-LG       10-20 hybs

25-50 hybs

DISPOSABLE POLYPROPYLENE CENTRIFUGE TUBES    

1.      Series A Tubes have standard snap-in caps

Cat #         Quantity

PP 141A

PP 142A

PP 143A

PP 144A   5 ml (13 x 75 mm) 125/box

8 ml (13 x 100 mm) 125/box

13 ml (17 x 95 mm) 75/box

20 ml (21 x 100 mm) 50/box

2.      Series B Tubes have Double-edge caps

Cat #         Quantity

PP 141B

PP 142B

PP 143B   5 ml (13 x 75 mm) 125/box

8 ml (13 x 100 mm) 125/box

13 ml (17 x 95 mm) 75/box

3.      Disposable Polypropylene Microfuge tubes

        Disposable Polypropylene Microfuge Tubes are tested in RNA, DNA and protein isolation using TRI Reagents, and withstand centrifugal forces of 12,000 g. The tubes fit eppendorf type centrifuges and have screw caps to reduce the hazard of phenol contamination. Made of ultra-clear polypropylene.

Cat #         Quantity

PP 131

PP 132      2 ml non-sterile 200/box

2 ml sterile 200/box

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